MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18738-1-AP

Synonyms

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

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Validation Data Gallery

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  WB analysis of mouse brain using 18738-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 18738-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 18738-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 18738-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18738-1-AP targets MAPK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 4
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC117216
• Gene Symbol: MAPK4
• Gene ID (NCBI): 5596
• RRID: AB_2878559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.