JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, canine and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28007-1-AP

Synonyms

c Jun N terminal kinase 3, FLJ12099, FLJ33785, JNK, JNK1, JNK1/2/3, JNK2, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, MAPK8, MAPK8/9/10, MAPK9, p493F12, p54bSAPK, PRKM10

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Validation Data Gallery

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  WB analysis using 28007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 28007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28007-1-AP (MAPK8/9/10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28007-1-AP (MAPK8/9/10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, MDCK cells, mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28007-1-AP targets JNK in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, canine samples.

• Tested Reactivity: Human, Mouse, canine
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag27823
• Full Name: mitogen-activated protein kinase 10
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 45-48 kDa, 54-57 kDa
• GenBank Accession Number: BC065516
• Gene Symbol: JNK3
• Gene ID (NCBI): 5602
• RRID: AB_2881035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JNK, also named MAPK10 (mitogen-activated protein kinase 10), belongs to MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is mainly expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. JNK has 3 isoforms of 48-53 kDa.

Publications

Species	Application	Title
mouse	WB	Int J Biol Macromol; VSIG4 inhibits RANKL-induced osteoclastogenesis by enhancing Nrf2-dependent antioxidant response against reactive oxygen species production; Authors - Jiansen Miao