MAP9 Polyclonal antibody

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  WB analysis of SH-SY5Y using 26078-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26078-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26078-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26078-1-AP (MAP9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26078-1-AP (MAP9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26078-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP9 antibody (26078-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26078-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Microtubule-associated protein 9, Aster-associated protein, Aster associated protein, ASAP

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

26078-1-AP targets MAP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP9 fusion protein Ag23446
• Full Name: microtubule-associated protein 9
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC146864
• Gene Symbol: MAP9
• Gene ID (NCBI): 79884
• RRID: AB_2880366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q49MG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
canine	IF	Front Cell Neurosci; Molecular characterization of MAP9 in the photoreceptor sensory cilia as a modifier in canine RPGRIP1-associated cone-rod dystrophy; Authors - Kei Takahashi
human	IHC,IF	Dev Cell; MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement; Authors - Michael V Tran
mouse	IF	EMBO J; Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium; Authors - Olivier Mercey