MAP7 Polyclonal antibody, PBS Only

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13446-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A549 using 13446-1-AP (same clone as 13446-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 13446-1-AP (same clone as 13446-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13446-1-AP (same clone as 13446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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Product Information

13446-1-PBS targets MAP7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP7 fusion protein Ag4276
• Full Name: microtubule-associated protein 7
• Calculated Molecular Weight: 749 aa, 84 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC025777
• Gene Symbol: MAP7
• Gene ID (NCBI): 9053
• RRID: AB_10644485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.