MAP4K3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP4K3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14702-1-AP

Synonyms

GLK, MAP4K3, MAPKKKK3, MEK kinase kinase 3, MEKKK 3, RAB8IPL1

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 14702-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14702-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14702-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, HeLa cells, human brain tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14702-1-AP targets MAP4K3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP4K3 fusion protein Ag6372
• Full Name: mitogen-activated protein kinase kinase kinase kinase 3
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC071579
• Gene Symbol: MAP4K3
• Gene ID (NCBI): 8491
• RRID: AB_10858917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

Publications

Species	Application	Title
mouse,rat	WB	Bioengineered; Mitogen-activating protein kinase kinase kinase kinase-3, inhibited by Astragaloside IV through H3 lysine 4 monomethylation, promotes the progression of diabetic nephropathy by inducing apoptosis.; Authors - Yuyan Fan ; KD Validated