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- KD/KO Validated
TAB2 Polyclonal antibody
TAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14410-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, C6 cells, human liver tissue, HepG2 cells, mouse liver tissue, rat liver tissue, Raji cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 17 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
Product Information
14410-1-AP targets TAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TAB2 fusion protein Ag5742 |
Full Name | mitogen-activated protein kinase kinase kinase 7 interacting protein 2 |
Calculated Molecular Weight | 76 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC035910 |
Gene Symbol | MAP3K7IP2 |
Gene ID (NCBI) | 23118 |
RRID | AB_2281638 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TAB2 antibody 14410-1-AP | Download protocol |
IHC protocol for TAB2 antibody 14410-1-AP | Download protocol |
IF protocol for TAB2 antibody 14410-1-AP | Download protocol |
IP protocol for TAB2 antibody 14410-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. | ||
Cell Biol Toxicol microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.
| ||
Front Immunol MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2. | ||
J Neuroinflammation miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses. | ||
Front Immunol Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex. |