MLK2 Polyclonal antibody

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  WB analysis using 29985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MLK2 Polyclonal Antibody for WB, ELISA

Cat No. 29985-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

MAP3K10, EC:2.7.11.25, Protein kinase MST, MST, Mixed lineage kinase 2

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29985-1-AP targets MLK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLK2 fusion protein Ag32419
• Full Name: mitogen-activated protein kinase kinase kinase 10
• Calculated Molecular Weight: 104KD
• Observed Molecular Weight: 104-115 kDa
• GenBank Accession Number: NM_002446
• Gene Symbol: MAP3K10
• Gene ID (NCBI): 4294
• RRID: AB_3086204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02779
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. MAP3K10 can be detected 104-115 kDa by phosphorylation (PMID: 23178452).

Publications

Species	Application	Title
rat	WB	Comb Chem High Throughput Screen; Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve; Authors - Wanjing Li