MEK5 Polyclonal antibody

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  WB analysis of HEK-293T using 15758-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15758-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15758-1-AP (MEK5 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK5 antibody (15758-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MEK5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15758-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MAP2K5, MKK5, MAPKK5, HsT17454, EC:2.7.12.2

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Tested Applications

• Positive WB detected in: HEK-293T cells, A431 cells, HeLa cells
• Positive IHC detected in: human heart tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15758-1-AP targets MEK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEK5 fusion protein Ag8351
• Full Name: mitogen-activated protein kinase kinase 5
• Calculated Molecular Weight: 448 aa, 50 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008838
• Gene Symbol: MEK5
• Gene ID (NCBI): 5607
• RRID: AB_2141275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13163
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Hyperthermia; Decrease in MAP3Ks expression enhances the cell death caused by hyperthermia.; Authors - Atsushi Enomoto
rat	WB	Pharm Biol; Injection of YiQiFuMai powder protects against heart failure via inhibiting p38 and ERK1/2 MAPKs activation.; Authors - Yongwei Nie