MAP2K3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP2K3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13898-1-AP

Synonyms

MEK3, MAPKK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3

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Validation Data Gallery

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  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, mouse skeletal muscle tissue, A549 cells, THP-1 cells, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below

Product Information

13898-1-AP targets MAP2K3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP2K3 fusion protein Ag4861
• Full Name: mitogen-activated protein kinase kinase 3
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC032478
• Gene Symbol: MAP2K3
• Gene ID (NCBI): 5606
• RRID: AB_10642959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP2K3, also named MKK3, is a member of the dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division, and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells; Authors - Ling Gu ; KD Validated
human	WB	Cell Mol Life Sci; HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress; Authors - Joan Sala-Gaston
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza ; KD Validated
mouse	WB	Mol Immunol; Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.; Authors - Libo Hou
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang