Filter: AllIHCIF-PFC (Intra)

IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
FC experiment of Neuro-2a using 67015-1-Ig (same clone as 67015-1-PBS)
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.
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1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

MAP2 Monoclonal antibody, PBS Only

MAP2 Monoclonal Antibody for IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 67015-1-PBS

Clone No.1C3E6

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67015-1-PBS targets MAP2 in IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	MAP2 fusion protein Ag11349 Predict reactive species
Full Name	microtubule-associated protein 2
Calculated Molecular Weight	200 kDa
GenBank Accession Number	BC038857
Gene Symbol	MAP2
Gene ID (NCBI)	4133
RRID	AB_2882331
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P11137
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.