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MAP1S Polyclonal antibody

MAP1S Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15695-1-AP

Synonyms

BPY2 interacting protein 1, BPY2IP1, C19orf5, MAP 1S, MAP1S, MAP8, VCY2 interacting protein 1, VCY2IP 1, VCY2IP1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15695-1-AP targets MAP1S in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP1S fusion protein Ag8315
Full Name microtubule-associated protein 1S
Calculated Molecular Weight 806 aa, 85 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC008806
Gene Symbol MAP1S
Gene ID (NCBI) 55201
RRIDAB_2137868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S.

Protocols

Product Specific Protocols
WB protocol for MAP1S antibody 15695-1-APDownload protocol
IHC protocol for MAP1S antibody 15695-1-APDownload protocol
IF protocol for MAP1S antibody 15695-1-APDownload protocol
IP protocol for MAP1S antibody 15695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Authors - Lei Wang
humanWB

EMBO Rep

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

Authors - Kohei Arasaki
humanWB

Cell Cycle

Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.

Authors - Junyu Wu
mouseIF

Front Synaptic Neurosci

Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction.

Authors - Yutaka Furutani
human,mouseWB,IP

Biochim Biophys Acta Mol Cell Res

Cryptic splicing events result in unexpected protein products from calpain-10 (CAPN10) cDNA

Authors - Yasuko Ono