MAP1S Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP1S Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15695-1-AP

Synonyms

BPY2 interacting protein 1, BPY2IP1, C19orf5, MAP 1S, MAP1S, MAP8, VCY2 interacting protein 1, VCY2IP 1, VCY2IP1

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Validation Data Gallery

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  WB analysis using 15695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15695-1-AP (MAP1S antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15695-1-AP

  WB result of MAP1S antibody (15695-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP1S transfected HeLa cells.

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  IP experiment of SH-SY5Y using 15695-1-AP

  IP result of anti-MAP1S (IP:15695-1-AP, 4ug; Detection:15695-1-AP 1:500) with SH-SY5Y cells lysate 1800ug.

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  IHC staining of human prostate cancer using 15695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15695-1-AP (MAP1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15695-1-AP (MAP1S antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15695-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15695-1-AP (MAP1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 15695-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAP1S antibody (15695-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 15695-1-AP

  Immunofluorescent analysis of Hela cells, using MAP1S antibody 15695-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15695-1-AP targets MAP1S in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP1S fusion protein Ag8315
• Full Name: microtubule-associated protein 1S
• Calculated Molecular Weight: 806 aa, 85 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC008806
• Gene Symbol: MAP1S
• Gene ID (NCBI): 55201
• RRID: AB_2137868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S.

Publications

Species	Application	Title
human	WB	Cell Res; Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.; Authors - Lei Wang
human	WB	EMBO Rep; MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.; Authors - Kohei Arasaki
human	WB	Cell Cycle; Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.; Authors - Junyu Wu
mouse	IF	Front Synaptic Neurosci; Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction.; Authors - Yutaka Furutani
human,mouse	WB,IP	Biochim Biophys Acta Mol Cell Res; Cryptic splicing events result in unexpected protein products from calpain-10 (CAPN10) cDNA; Authors - Yasuko Ono