LC3A-Specific Polyclonal antibody
LC3A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18722-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
18722-1-AP targets LC3A-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | microtubule-associated protein 1 light chain 3 alpha |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 15-18 kDa |
GenBank Accession Number | NM_032514 |
Gene Symbol | LC3 |
Gene ID (NCBI) | 84557 |
RRID | AB_10597104 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LC3A, also named as MAP1LC3A, LC3, MAP1ALC3 and MAP1BLC3, belongs to the MAP1 LC3 family. LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B which are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. (PMID:11060023) MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.(PMID:12740394 ) This antibody is specific to LC3A.It recognize both LC3A-I and LC3A-II.
Protocols
Product Specific Protocols | |
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WB protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
IHC protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
IF protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats. | ||
Oxid Med Cell Longev Imbalance of Autophagy and Apoptosis Induced by Oxidative Stress May Be Involved in Thyroid Damage Caused by Sleep Deprivation in Rats. | ||
Front Cell Dev Biol Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes. | ||
J Cell Mol Med Connexin 31.1 degradation requires the Clathrin-mediated autophagy in NSCLC cell H1299. | ||
J Immunol Cutting Edge: Phagosome-associated Autophagosomes Containing Antigens and Proteasomes Drive TAP-Independent Cross-Presentation | ||
Mar Drugs 7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway. |