LC3A-Specific Polyclonal antibody

LC3A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18722-1-AP

Synonyms

LC3, MAP1LC3A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, LC3A

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Validation Data Gallery

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  WB analysis of mouse brain using 18722-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18722-1-AP (LC3A-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18722-1-AP (LC3A-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18722-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18722-1-AP (LC3A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 18722-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18722-1-AP (LC3A-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18722-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18722-1-AP (LC3A-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

18722-1-AP targets LC3A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: microtubule-associated protein 1 light chain 3 alpha
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: NM_032514
• Gene Symbol: LC3
• Gene ID (NCBI): 84557
• RRID: AB_10597104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LC3A, also named as MAP1LC3A, LC3, MAP1ALC3 and MAP1BLC3, belongs to the MAP1 LC3 family. LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B which are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. (PMID:11060023) MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.(PMID:12740394 ) This antibody is specific to LC3A.

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats.; Authors - Yongmei Li
rat	WB	Oxid Med Cell Longev; Imbalance of Autophagy and Apoptosis Induced by Oxidative Stress May Be Involved in Thyroid Damage Caused by Sleep Deprivation in Rats.; Authors - Yongmei Li
zebrafish	WB	Front Cell Dev Biol; Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes.; Authors - Xiaoqing Lv
human	WB	J Cell Mol Med; Connexin 31.1 degradation requires the Clathrin-mediated autophagy in NSCLC cell H1299.; Authors - Xingli Zhu
mouse	IF	J Immunol; Cutting Edge: Phagosome-associated Autophagosomes Containing Antigens and Proteasomes Drive TAP-Independent Cross-Presentation; Authors - Debrup Sengupta
human	WB	Mar Drugs; 7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.; Authors - Tsung-Chang Tsai