MAP1A Polyclonal antibody

MAP1A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55062-1-AP

Synonyms

LC2, MAP1A, MAP1A protein, MAP1L, MTAP1A

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Validation Data Gallery

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  IHC staining of mouse cerebellum using 55062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55062-1-AP (MAP1A antibody) at dilution of 1:500 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 55062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55062-1-AP (MAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 55062-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55062-1-AP (MAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55062-1-AP targets MAP1A in IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: microtubule-associated protein 1A
• Calculated Molecular Weight: 305 kDa
• GenBank Accession Number: NM_002373
• Gene Symbol: MAP1A
• Gene ID (NCBI): 4130
• RRID: AB_2918355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The assembly of microtubules, an essential step in neurogenesis, is modulated by the family of microtubule-associated proteins (MAPs). These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B , and MAP2 , and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1A is the largest brain MAP and expressed prominently in dendrites and to a lesser extent in cell bodies and axons . This protein is involved in differentiation of axons and dendrites as well as maintenance of the microtubule cytoskeleton in mature neurons.