MAOB Monoclonal antibody, PBS Only

MAOB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12F3

Cat no : 66107-1-PBS

Synonyms

MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B

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Validation Data Gallery

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  WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 66107-1-Ig (same clone as 66107-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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Product Information

66107-1-PBS targets MAOB in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAOB fusion protein Ag17918
• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).