MANEA Polyclonal antibody

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  WB analysis of HEK-293 using 21296-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21296-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:100 incubated at 4 degree celsius over night.

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  IHC staining of human breast cancer using 21296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 21296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HEK-293 using 21296-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MANEA antibody (21296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21296-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21296-1-AP targets MANEA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MANEA fusion protein Ag15847
• Full Name: mannosidase, endo-alpha
• Calculated Molecular Weight: 462 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC146671
• Gene Symbol: MANEA
• Gene ID (NCBI): 79694
• RRID: AB_2878836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5SRI9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.