MAN2C1 Polyclonal antibody

MAN2C1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19189-1-AP

Synonyms

Alpha mannosidase 2C1, Alpha mannosidase 6A8B, MAN2C1, MAN6A8, MANA, MANA1

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Validation Data Gallery

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  WB analysis using 19189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19189-1-AP (MAN2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19189-1-AP targets MAN2C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAN2C1 fusion protein Ag6284
• Full Name: mannosidase, alpha, class 2C, member 1
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC050550
• Gene Symbol: MAN2C1
• Gene ID (NCBI): 4123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha mannosidase 2C1(MAN2C1) an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol. MAN2C1 cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmatic free oligosaccharides generated by N-glycoprotein degradation pathways (PMID: 16848760). Impaired catabolism of free oligosaccharides due to MAN2C1 variants causes a neurodevelopmental disorder (PMID: 35045343).