MAN2B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAN2B2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17697-1-AP

Synonyms

KIAA0935, MAN2B2

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Validation Data Gallery

• 

  WB analysis of mouse testis using 17697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17697-1-AP (MAN2B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 17697-1-AP

  WB result of MAN2B2 antibody (17697-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAN2B2 transfected NIH/3T3 cells.

• 

  WB analysis of mouse lung using 17697-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17697-1-AP (MAN2B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse lung tissue, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17697-1-AP targets MAN2B2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAN2B2 fusion protein Ag11952
• Full Name: mannosidase, alpha, class 2B, member 2
• Calculated Molecular Weight: 1009 aa, 114 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC033307
• Gene Symbol: MAN2B2
• Gene ID (NCBI): 23324
• RRID: AB_2139566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter