MAN2A1 Polyclonal antibody

MAN2A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 21132-1-AP

Synonyms

Alpha mannosidase 2, AMan II, Golgi alpha mannosidase II, GOLIM7, Man II, MAN2A1, MANA2, MANII

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Validation Data Gallery

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  IHC staining of human stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HeLa using 21132-1-AP

  Immunofluorescent analysis of HeLa cells using 21132-1-AP (MAN2A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human stomach tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAN2A1 fusion protein Ag15536
• Full Name: mannosidase, alpha, class 2A, member 1
• Calculated Molecular Weight: 1144 aa, 131 kDa
• GenBank Accession Number: BC142656
• Gene Symbol: MAN2A1
• Gene ID (NCBI): 4124
• RRID: AB_2878816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-01-2020)

This antibody labels clean doublet bands around the expected molecular weight.

• Applications: Western Blot