MAML1 Polyclonal antibody

MAML1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55493-1-AP

Synonyms

KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1

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Validation Data Gallery

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  WB analysis using 55493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mastermind-like 1 (Drosophila)
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108-120 kDa
• GenBank Accession Number: NM_014757
• Gene Symbol: MAML1
• Gene ID (NCBI): 9794
• RRID: AB_11182286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.

Publications

Species	Application	Title
human	WB,CoIP	Int J Biol Sci; Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.; Authors - Yanzhu Shi