MAK Polyclonal antibody

MAK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13638-1-AP

Synonyms

MAK

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Validation Data Gallery

• 

  WB analysis of PC-3 using 13638-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13638-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13638-1-AP targets MAK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAK fusion protein Ag4571
• Full Name: male germ cell-associated kinase
• Calculated Molecular Weight: 623 aa, 71 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC039825
• Gene Symbol: MAK
• Gene ID (NCBI): 4117
• RRID: AB_10598167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	J Biol Chem; Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.; Authors - Ran Li