MAGT1 Polyclonal antibody

MAGT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27994-1-AP

Synonyms

bA217H1.1, DKFZp564K142, FLJ14726, IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244

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Validation Data Gallery

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  WB analysis using 27994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27994-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 27994-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 27994-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, HuH-7 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27994-1-AP targets MAGT1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGT1 fusion protein Ag27416
• Full Name: magnesium transporter 1
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC060842
• Gene Symbol: MAGT1
• Gene ID (NCBI): 84061
• RRID: AB_2881033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients' cells by western blot or immunofluorescent cell surface staining (Nature 475, 471-6). 27994-1-AP antibody detects the glycosylated protein around 45-50 kDa in SDS-PAGE. (PMID: 28720733, 27383987)

Publications

Species	Application	Title
human,mouse	WB	Int J Mol Med; Transcription factor KLF16 activates MAGT1 to regulate the tumorigenesis and progression of breast cancer.; Authors - Lin Li