MAGT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAGT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 17430-1-AP

Synonyms

magnesium transporter 1, Implantation-associated protein, IAP, IAG2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1

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Validation Data Gallery

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  WB analysis using 17430-1-AP

  Mouse cerebellum lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17430-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17430-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 17430-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17430-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17430-1-AP (MAGT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17430-1-AP

  Immunofluorescent analysis of HepG2 cells, using 17430-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of C2C12 using 17430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MAGT1 antibody (17430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, COLO 320 cells, HEK-293 cells, mouse colon tissue, mouse heart tissue, rat cerebellum tissue
• Positive IHC detected in: human liver tissue, human colon cancer tissue, human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IF: See 5 publications below

Product Information

17430-1-AP targets MAGT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGT1 fusion protein Ag11496
• Full Name: magnesium transporter 1
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC060842
• Gene Symbol: MAGT1
• Gene ID (NCBI): 84061
• RRID: AB_2138982
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.

Publications

Species	Application	Title
human	WB,IF	Nature; Second messenger role for Mg2+ revealed by human T-cell immunodeficiency.; Authors - Li Feng-Yen FY
human	WB	Nature; Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.; Authors - Caleb D Marceau ; KO Validated
rat	WB,IF	Adv Sci (Weinh); A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration.; Authors - Sihan Lin
rat	WB,IF	Small; Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold; Authors - Zuyun Yan
human	WB, IF	J Cell Biol; Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Natalia (Verified Customer) (09-26-2019)

To my knowledge, this one is the only one commercially available antibody that works well for WB application to detect MagT1. It cross-reacts with TUSC3 a little, only because these are highly homological proteins.

• Applications: Western Blot, Immunofluorescence,

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