MAGOH Monoclonal antibody

MAGOH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, MOUSE, RAT, RABBIT

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6B9

Cat no : 68293-1-Ig

Synonyms

MAGOH, MAGOHA, Protein mago nashi homolog

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Validation Data Gallery

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  WB analysis using 68293-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68293-1-Ig (MAGOH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68293-1-Ig targets MAGOH in WB, ELISA applications and shows reactivity with Human, MOUSE, RAT, RABBIT samples.

• Tested Reactivity: Human, MOUSE, RAT, RABBIT
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAGOH fusion protein Ag3004
• Full Name: mago-nashi homolog, proliferation-associated (Drosophila)
• Calculated Molecular Weight: 146 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC018211
• Gene Symbol: MAGOH
• Gene ID (NCBI): 4116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.