MAGI3 Recombinant antibody

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  WB analysis using 84771-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84771-4-RR (MAGI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 84771-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGI3 antibody (84771-4-RR, Clone: 242212C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84771-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84771-4-RR against Human MAGI3 were performed. The affinity constant is 0.752 nM.

MAGI3 Uni-rAb^TM Recombinant Antibody for WB, IF-P, ELISA

Cat No. 84771-4-RR

Clone No.242212C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634, 242212C9

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84771-4-RR targets MAGI3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAGI3 fusion protein Ag18394
• Full Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
• Calculated Molecular Weight: 1481 aa, 163 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC130409
• Gene Symbol: MAGI3
• Gene ID (NCBI): 260425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5TCQ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.