MAGED2 Polyclonal antibody, PBS Only

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15252-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15252-1-AP (same clone as 15252-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 15252-1-AP (same clone as 15252-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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Product Information

15252-1-PBS targets MAGED2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGED2 fusion protein Ag6828
• Full Name: melanoma antigen family D, 2
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000304
• Gene Symbol: MAGED2
• Gene ID (NCBI): 10916
• RRID: AB_2138557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.