MAGEB4 Polyclonal antibody

MAGEB4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12786-2-AP

Synonyms

Melanoma-associated antigen B4, MAGE-B4 antigen

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Validation Data Gallery

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  WB analysis using 12786-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12786-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12786-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12786-2-AP

  IP result of anti-MAGEB4 (IP:12786-2-AP, 4ug; Detection:12786-2-AP 1:300) with K-562 cells lysate 4000 ug.

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  IF Staining of HepG2 using 12786-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB4 antibody (12786-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:600-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12786-2-AP targets MAGEB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEB4 fusion protein Ag3502
• Full Name: melanoma antigen family B, 4
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC032852
• Gene Symbol: MAGEB4
• Gene ID (NCBI): 4115
• RRID: AB_2138418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.