MAGEB3 Polyclonal antibody

MAGEB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18000-1-AP

Synonyms

CT3.5, MAGE B3 antigen, MAGEB3, melanoma antigen family B, 3, Melanoma associated antigen B3

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Validation Data Gallery

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  WB analysis using 18000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18000-1-AP (MAGEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 18000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18000-1-AP targets MAGEB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEB3 fusion protein Ag12476
• Full Name: melanoma antigen family B, 3
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC074756
• Gene Symbol: MAGEB3
• Gene ID (NCBI): 4114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. MAGEB3, also named CT3.5, is a member of the MAGEB gene family.