MAGEA8 Polyclonal antibody

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  WB analysis of A375 using 15045-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15045-1-AP (MAGEA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15045-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 15045-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A375 using 15045-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 15045-1-AP (MAGEA8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15045-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Melanoma-associated antigen 8, MAGE-8 antigen, MAGE8, CT1.8, Cancer/testis antigen 1.8

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15045-1-AP targets MAGEA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA8 fusion protein Ag6984
• Full Name: melanoma antigen family A, 8
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC002455
• Gene Symbol: MAGEA8
• Gene ID (NCBI): 4107
• RRID: AB_2138289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.