MAGEA4 Recombinant antibody, PBS Only

MAGEA4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241558B10

Cat no : 84248-6-PBS

Synonyms

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

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Validation Data Gallery

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  WB analysis using 84248-6-RR (same clone as 84248-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 84248-6-RR (same clone as 84248-6-PBS)

  human testis tissue was subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 84248-6-RR (same clone as 84248-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 84248-6-RR (same clone as 84248-6-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 84248-6-PBS is MAGEA4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAGEA4 fusion protein Ag3193
• Full Name: melanoma antigen family A, 4
• Calculated Molecular Weight: 317 aa, 35 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017723
• Gene Symbol: MAGEA4
• Gene ID (NCBI): 4103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.