MAGEA11 Polyclonal antibody

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  WB analysis of A375 using 15474-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15474-1-AP (MAGEA11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 15474-1-AP

  Immunofluorescent analysis of A375 cells using 15474-1-AP (MAGEA11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEA11 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15474-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15474-1-AP targets MAGEA11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA11 fusion protein Ag7778
• Full Name: melanoma antigen family A, 11
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa, 44 kDa
• GenBank Accession Number: BC004479
• Gene Symbol: MAGEA11
• Gene ID (NCBI): 4110
• RRID: AB_2137973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43364
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Sci Rep; Integrated analysis of single-cell and bulk RNA-sequencing reveals a novel signature based on NK cell marker genes to predict prognosis and immunotherapy response in gastric cancer; Authors - Jian-Rong Sun