MAGEA10 Polyclonal antibody

MAGEA10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15295-1-AP

Synonyms

Cancer/testis antigen 1.10, CT1.10, MAGE 10 antigen, MAGE10, MAGEA10, melanoma antigen family A, 10, Melanoma associated antigen 10

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Validation Data Gallery

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  WB analysis of A375 using 15295-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15295-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A375 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15295-1-AP targets MAGEA10 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA10 fusion protein Ag7540
• Full Name: melanoma antigen family A, 10
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 62 kDa, 41 kDa
• GenBank Accession Number: BC004105
• Gene Symbol: MAGEA10
• Gene ID (NCBI): 4109
• RRID: AB_2137968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	BMC Cancer; Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells.; Authors - Xiao Song