MAGEA1 Polyclonal antibody, PBS Only

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  WB analysis of A375 using 55105-1-AP (same clone as 55105-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 55105-1-AP (same clone as 55105-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 55105-1-AP (same clone as 55105-1-PBS)

  Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55105-1-PBS in a different storage buffer formulation.

MAGEA1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 55105-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

55105-1-PBS targets MAGEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_004988
• Gene Symbol: MAGEA1
• Gene ID (NCBI): 4100
• RRID: AB_10859942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43355
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.