MAGEA1 Polyclonal antibody

MAGEA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12233-1-AP

Synonyms

Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1

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Validation Data Gallery

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  WB analysis of A375 using 12233-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12233-1-AP (MAGEA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12233-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 12233-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12233-1-AP (MAGEA1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12233-1-AP targets MAGEA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA1 fusion protein Ag2871
• Full Name: melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• Calculated Molecular Weight: 309 aa, 34 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC017555
• Gene Symbol: MAGEA1
• Gene ID (NCBI): 4100
• RRID: AB_2250080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.