MAG Recombinant antibody, PBS Only

• 

  WB analysis of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86097-1-RR (MAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

MAG Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86097-1-PBS

Clone No.250555A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Product Information

86097-1-PBS targets MAG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAG fusion protein Eg2975
• Full Name: myelin associated glycoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_002361.3
• Gene Symbol: MAG
• Gene ID (NCBI): 4099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20916-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.