MAEL Polyclonal antibody

• Featured Product
• KD/KO Validated

MAEL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, RIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26666-1-AP

Synonyms

MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1

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Validation Data Gallery

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  WB analysis of rat testis using 26666-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26666-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 26666-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 26666-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 26666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26666-1-AP (MAEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

26666-1-AP targets MAEL in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAEL fusion protein Ag24524
• Full Name: maelstrom homolog (Drosophila)
• Calculated Molecular Weight: 434 aa, 49 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC028595
• Gene Symbol: MAEL
• Gene ID (NCBI): 84944
• RRID: AB_2880595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IF,RIP	Andrology; The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia; Authors - Yu-Sheng Cheng ; KD Validated
mouse	WB	J Cell Mol Med; Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice; Authors - Xinying Bi