Multi-rAb™ MAEA Multi-Recombinant antibody

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  WB analysis using RMX00005

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00005 (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using RMX00005

  IP result of anti-MAEA (IP:RMX00005, 4ug; Detection:RMX00005 1:40000) with HepG2 cells lysate 2400 ug.

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  IF Staining of HeLa using RMX00005

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (RMX00005) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

MAEA Multi-Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. RMX00005

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

HLC 10, EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, U2OS cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00005 targets MAEA in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: macrophage erythroblast attacher
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001225
• Gene Symbol: MAEA
• Gene ID (NCBI): 10296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q7L5Y9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).