MAD2L2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MAD2L2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12683-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12683-1-AP (same clone as 12683-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 12683-1-AP (same clone as 12683-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12683-1-AP (same clone as 12683-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12683-1-AP (same clone as 12683-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 12683-1-AP (same clone as 12683-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12683-1-AP (same clone as 12683-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 12683-1-AP (same clone as 12683-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

  IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 12683-1-AP (same clone as 12683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 12683-1-AP (same clone as 12683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

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Product Information

12683-1-PBS targets MAD2L2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAD2L2 fusion protein Ag3373
• Full Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015244
• Gene Symbol: MAD2L2
• Gene ID (NCBI): 10459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.