MAD2L1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MAD2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F5F12

Cat no : 66014-1-PBS

Synonyms

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 66014-1-Ig (same clone as 66014-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66014-1-Ig (same clone as 66014-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66014-1-Ig (same clone as 66014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66014-1-PBS targets MAD2L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAD2L1 fusion protein Ag17512
• Full Name: MAD2 mitotic arrest deficient-like 1 (yeast)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC000356
• Gene Symbol: MAD2L1
• Gene ID (NCBI): 4085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.