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M-cadherin Recombinant antibody, PBS Only

M-cadherin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241837C10

Cat no : 84440-5-PBS

Synonyms

CDH15, CDH14, Cadherin-15, Cadherin-14, Cadherin 15

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using M-cadherin Recombinant antibody (84440-5-RR)

    WB analysis of mouse cerebellum using 84440-5-RR (same clone as 84440-5-PBS)

    mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 84440-5-RR (CDH15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using M-cadherin Recombinant antibody (84440-5-RR)

    IF Staining of C2C12 using 84440-5-RR (same clone as 84440-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CDH15 antibody (84440-5-RR, Clone: 241837C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84440-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84440-5-RR

    Affinity and Kinetic Characterization of 84440-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84440-5-RR against Human M-cadherin were performed. The affinity constant is below 1 pM.

Product Information

84440-5-PBS targets M-cadherin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen M-cadherin fusion protein Ag25763
Full Name cadherin 15, type 1, M-cadherin (myotubule)
Calculated Molecular Weight 89 kDa
Observed Molecular Weight90-130 kDa
GenBank Accession NumberBC008951
Gene Symbol M-cadherin
Gene ID (NCBI) 1013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).