Leptin Recombinant antibody

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  WB analysis of rat adipose using 83655-3-RR

  Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83655-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

Leptin {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83655-3-RR

Clone No.240368E6

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Synonyms

Lep, OB, 240368E6

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Tested Applications

• Positive WB detected in: rat adipose tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83655-3-RR targets Leptin in WB, IHC, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: leptin
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 15-16 kDa
• GenBank Accession Number: NM_013076.3
• Gene Symbol: Leptin
• Gene ID (NCBI): 25608
• RRID: AB_3671263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50596
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.