Lactotransferrin/LTF Recombinant antibody, PBS Only

Lactotransferrin/LTF Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240280E2

Cat no : 83409-5-PBS

Synonyms

Lactoferrin, LTF, HLF2, Growth-inhibiting protein 12, GIG12

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Validation Data Gallery

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  WB analysis of human saliva using 83409-5-RR (same clone as 83409-5-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 83409-5-RR (Lactotransferrin/LTF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83409-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83409-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83409-5-RR against Human Lactotransferrin/LTF were performed. The affinity constant is 0.896 nM.

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Product Information

83409-5-PBS targets Lactotransferrin/LTF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lactotransferrin
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 30 kDa, 51 kDa, 80 kDa
• GenBank Accession Number: NM_002343.6
• Gene Symbol: LTF
• Gene ID (NCBI): 4057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).