LZTS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LZTS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20878-1-AP

Synonyms

Fez1, F37/esophageal cancer-related gene-coding leucine-zipper motif, F37

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Validation Data Gallery

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  WB analysis using 20878-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20878-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20878-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20878-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20878-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 20878-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20878-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20878-1-AP

  IP result of anti-LZTS1 (IP:20878-1-AP, 3ug; Detection:20878-1-AP 1:500) with mouse brain tissue lysate 7500ug.

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  IF Staining of HepG2 using 20878-1-AP

  Immunofluorescent analysis of HepG2 cells, using LZTS1 antibody 20878-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, human testis tissue, mouse ovary tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

20878-1-AP targets LZTS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LZTS1 fusion protein Ag14977
• Full Name: leucine zipper, putative tumor suppressor 1
• Calculated Molecular Weight: 596 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC075007
• Gene Symbol: LZTS1
• Gene ID (NCBI): 11178
• RRID: AB_10693683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.; Authors - T Kawaue ; KO Validated
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen