LYSMD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

LYSMD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24313-1-AP

Synonyms

LYSMD3

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Validation Data Gallery

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  WB analysis of Neuro-2a using 24313-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24313-1-AP (LYSMD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24313-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24313-1-AP (LYSMD3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 24313-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24313-1-AP (LYSMD3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Neuro-2a cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 24313-1-AP is LYSMD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LYSMD3 fusion protein Ag19430
• Full Name: LysM, putative peptidoglycan-binding, domain containing 3
• Calculated Molecular Weight: 306 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC136741
• Gene Symbol: LYSMD3
• Gene ID (NCBI): 116068
• RRID: AB_2879490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYSMD3 also termed as LysM and putative peptidoglycan binding domain containing protein 3 is a 306 amino acid single-pass membrane protein that contains one LysM repeat. LysM domain encoding genes may play a role in brain and in nervous system development and functioning. LYSMD3, shows a high level of central nervous expression during embryogenesis and is also, therefore, a good candidate gene for other central nervous system (CNS) symptoms, such as psychomotor retardation, brain anomalies and muscular hypotonia of the 5q14.3 microdeletion syndrome.

Publications

Species	Application	Title
		J Biol Chem; LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens.; Authors - Christine C Yokoyama
human	WB	Mol Biol Cell; Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response.; Authors - Yevgeniy V Serebrenik
human	WB	Cell Rep; LYSMD3: A mammalian pattern recognition receptor for chitin.; Authors - Xin He ; KO Validated