LYPD1 Polyclonal antibody

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  WB analysis using 11415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11415-1-AP (LYPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 11415-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11415-1-AP (LYPD1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LYPD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11415-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11415-1-AP targets LYPD1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LYPD1 fusion protein Ag1965
• Full Name: LY6/PLAUR domain containing 1
• Calculated Molecular Weight: 141 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017318
• Gene Symbol: LYPD1
• Gene ID (NCBI): 116372
• RRID: AB_3085372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N2G4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LY6/PLAUR domain containing 1 (LYPD1) is a highly conserved glycosylphosphatidylinositol (GPI)-anchored protein. LYPD1 contains one cysteine-rich extracellular leukocyte antigen-6 (Ly6)/uPAR (PLAUR) domain followed by signal sequence that triggers posttranslational GPI-anchor addition incorporating mature, LYPD1 into the plasma membrane. LYPD1 is believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. LYPD1 increases receptor desensitization in vitro and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. LYPD1 has 4 isoforms produced by alternative splicing (PMID: 33536191).