LY6H Polyclonal antibody

LY6H Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23933-1-AP

Synonyms

Ly 6H, LY6H, Lymphocyte antigen 6H

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Validation Data Gallery

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  WB analysis of Raji using 23933-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23933-1-AP (LY6H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23933-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23933-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 23933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23933-1-AP (LY6H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 23933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23933-1-AP (LY6H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23933-1-AP targets LY6H in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LY6H fusion protein Ag20762
• Full Name: lymphocyte antigen 6 complex, locus H
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC028894
• Gene Symbol: LY6H
• Gene ID (NCBI): 4062
• RRID: AB_2879366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LY6H is highly expressed in particular subdivisions of human brain and also in MOLT-3 and -4 acute lymphoblastic leukemia cells. It has some isoforms with MW 14-16 kDa.