LY6E Polyclonal antibody

• Featured Product
• KD/KO Validated

LY6E Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22144-1-AP

Synonyms

RIG-E, RIGE, Retinoic acid-induced gene E protein, Ly-6E, Ly 6E

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Validation Data Gallery

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  WB analysis using 22144-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22144-1-AP (LY6E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 22144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22144-1-AP (LY6E Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 22144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22144-1-AP (LY6E Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 22144-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22144-1-AP (LY6E Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 22144-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22144-1-AP (LY6E Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human tonsillitis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LY6E fusion protein Ag17573
• Full Name: lymphocyte antigen 6 complex, locus E
• Calculated Molecular Weight: 131 aa, 14 kDa
• Observed Molecular Weight: 12-16 kDa
• GenBank Accession Number: BC119708
• Gene Symbol: LY6E
• Gene ID (NCBI): 4061
• RRID: AB_2879007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.

Publications

Species	Application	Title
human	WB,IF	Lab Chip; LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier; Authors - Dan Liu
human	WB,IHC	Carcinogenesis; α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.; Authors - Qian Zhang
human	WB	J Virol; LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.; Authors - Xuesen Zhao ; KD Validated
human	WB	J Virol; Characterization of CCoV-HuPn-2018 spike protein-mediated viral entry; Authors - Yongmei Liu