|Positive WB detected in||mouse kidney tissue|
|Positive IHC detected in||human tonsillitis tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, Mouse samples.
|Tested Reactivity||human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LY6E fusion protein Ag17573|
|Full Name||lymphocyte antigen 6 complex, locus E|
|Calculated molecular weight||131 aa, 14 kDa|
|Observed molecular weight||12-16 kDa|
|GenBank accession number||BC119708|
|Gene ID (NCBI)||4061|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.
LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier
α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.
LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.