LUC7L2 Polyclonal antibody

• Featured Product
• KD/KO Validated

LUC7L2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24202-1-AP

Synonyms

LUC7B2, LUC7 like 2 (S. cerevisiae), CGI-74, CGI-59, CGI 74

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Validation Data Gallery

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  WB analysis of K-562 using 24202-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24202-1-AP

  WB result of LUC7L2 antibody (24202-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L2 transfected HeLa cells.

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  WB analysis of HeLa using 24202-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 24202-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using LUC7L2 antibody (24202-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24202-1-AP targets LUC7L2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LUC7L2 fusion protein Ag21135
• Full Name: LUC7-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 392 aa, 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC017163
• Gene Symbol: LUC7L2
• Gene ID (NCBI): 51631
• RRID: AB_2879455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing, and promoted its nonsense-mediated decay, leading to its downregulation at the protein level (PMID:34155193). Moreover, LUC7L2 as a component of the U1 snRNP capable of reprogramming cellular metabolism through changes in alternative pre-mRNA splicing (PMID:33961773).