LTF Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LTF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10933-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10933-1-AP (same clone as 10933-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human pancreas using 10933-1-AP (same clone as 10933-1-PBS)

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10933-1-AP (same clone as 10933-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10933-1-AP (same clone as 10933-1-PBS)

  IP result of anti-LTF (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

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Product Information

10933-1-PBS targets LTF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTF fusion protein Ag1379
• Full Name: lactotransferrin
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 30,51,80 kDa
• GenBank Accession Number: BC015822
• Gene Symbol: LTF
• Gene ID (NCBI): 4057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).