Lactotransferrin/LTF antibody (10933-1-PBS)

Filter: AllWBIPIHC

WB analysis using 10933-1-AP (same clone as 10933-1-PBS)
human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10933-1-AP (same clone as 10933-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
WB analysis of human pancreas using 10933-1-AP (same clone as 10933-1-PBS)
human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 10933-1-AP (same clone as 10933-1-PBS)
IP result of anti-LTF (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

This product is currently not available for sale.

Tested Applications
Product Information

Product Information

10933-1-PBS targets Lactotransferrin/LTF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lactotransferrin/LTF fusion protein Ag1379 Predict reactive species
Full Name	lactotransferrin
Calculated Molecular Weight	80 kDa
Observed Molecular Weight	30,51,80 kDa
GenBank Accession Number	BC015822
Gene Symbol	LTF
Gene ID (NCBI)	4057
RRID	AB_2139227
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02788
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).