LTBP2 Polyclonal antibody

• 

  IP experiment of U2OS using 28824-1-AP

  IP result of anti-LTBP2 (IP:28824-1-AP, 4ug; Detection:28824-1-AP 1:600) with U2OS cells lysate 1840 ug.

• 

  IHC staining of mouse lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 28824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LTBP2 antibody (28824-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

LTBP2 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 28824-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

C14orf141, LTBP3, Latent-transforming growth factor beta-binding protein 2, LTBP 2, LTBP-2

---

Tested Applications

• Positive IP detected in: U2OS cells
• Positive IHC detected in: mouse lung tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28824-1-AP targets LTBP2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTBP2 fusion protein Ag29737
• Full Name: latent transforming growth factor beta binding protein 2
• Calculated Molecular Weight: 1821 aa, 195 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: BC078659
• Gene Symbol: LTBP2
• Gene ID (NCBI): 4053
• RRID: AB_3669670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14767
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LTBP2, an extracellular glycoprotein mapping to chromosome 14q24, belongs to a family of latent TGF-β binding proteins (LTBPs) that regulates a well-known growth factor, TGF-β. LTBP2 is an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCEbinding Protein. (PMID: 17581631 )It may play an integral structural role in elastic-fiber architectural organization and/or assembly. LTBP2 associates covalently with small latent TGF-beta complex via Repeat B and Repeat C. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). [MIM:613086] LTBP2 is a role in the structural stability of ciliary zonules, and growth and development of lens.(PMID: 20617341) LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma (PMID: 20179738 ) . LTBP2 is a biomarker of heart failure. LTBP2 has a molecular weight of 260 kDa (PMID: 20878956), and even larger molecular weights of 290 and 310 kDa were detected in tissue extracts. (PMID: 8524260)