LTBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 26855-1-AP

Synonyms

LTBP 1, LTBP1

---

Validation Data Gallery

• 

  WB analysis of human plasma using 26855-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26855-1-AP (LTBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26855-1-AP (LTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26855-1-AP is LTBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTBP1 fusion protein Ag25392
• Full Name: latent transforming growth factor beta binding protein 1
• Calculated Molecular Weight: 1721 aa, 187 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC130289
• Gene Symbol: LTBP1
• Gene ID (NCBI): 4052
• RRID: AB_2880658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein which belongs to the family of latent TGF-beta binding proteins (LTBPs). LTBP1 is essential for TGF-beta folding, secretion, matrix localization and activation. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular matrix components, such as fibrillin. We got 150-240 kDa in western blotting due to protein glycosylation of LTBP1 (PMID: 18086923, PMID: 9923648 ).

Publications

Species	Application	Title
human	IF	Cell Rep; Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis; Authors - Jose Verdezoto Mosquera
human	WB,IHC	J Transl Med; LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.; Authors - Rui Cai ; KD Validated
human	WB	Cell Death Discov; ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency.; Authors - Mingqing Cui
human,mouse	IHC	medRxiv; Secreted protein profiling of human aortic smooth muscle cells identifies vascular disease associations; Authors - Rédouane Aherrahrou
human	WB,IF	Adv Mater; Engineered Surfaces that Promote Capture of Latent Proteins to Facilitate Integrin-Mediated Mechanical Activation of Growth Factors; Authors - Udesh Dhawan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Udesh (Verified Customer) (10-05-2022)

The Ab was used at 1:1000 dilution in NFDM overnight 4 degrees and detected a band close to 220 kD in 20ug cell lysate. Also tested for for positive IF staining at 1:250 dilution in 2% BSA overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Bone Marrow MSCs

LTBP1 Antibody Western Blot validation (1:1000 dilution) in Bone Marrow MSCs (Cat no:26855-1-AP)